Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator

Femtosecond laser pulses can be used to selectively disrupt and dissect intracellular organelles. We report on disruption of mitochondria in living HeLa cells using a femtosecond laser oscillator with a repetition rate of 76 MHz. We studied the laser parameters used for disruption. The longterm viability of the cells after disruption of a single mitochondrion was confirmed by the observation of cell division, indicating that intracellular disruption of organelles using a femtosecond laser oscillator can be performed without compromising the long-term cell viability.Shimada T., Watanabe W., Matsunaga S., et al. Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator. Optics Express, 13, 24, 9869. https://doi.org/10.1364/OPEX.13.009869