Species and tissues specific differentiation of processed animal proteins in aquafeeds using proteomics tools

Significance: To guarantee the safe use of processed animal protein (PAP) in aquafeeds efficient PAP detection and monitoring tools are required. The present study investigated and compared various proteomics workflows and shows that the application of shotgun proteomics in combination with direct comparison of spectral libraries provides for the desired species and tissue specific classification of this heat sterilized and pressure treated (>= 133 degrees C, at 3 bar for 20 min) protein feed ingredient. (C) 2016 Elsevier B.V. All rights reserved.Proteomic