Diabetic Nephropathy Alters the Distribution of Circulating Angiogenic MicroRNAs Among Extracellular Vesicles, HDL, and Ago-2
- Florijn, B.W.
- Duijs, J.M.G.J.
- Levels, J.H.
- Dallinga-Thie, G.M.
- Wang, Y.N.
- Boing, A.N.
- Yuana, Y.
- Stam, W.
- Limpens, R.W.A.L.
- Au, Y.W.
- Nieuwland, R.
- Rabelink, T.J.
- Reinders, M.E.J.
- Zonneveld, A.J. van
- Bijkerk, R.
DOIAbstract
Previously, we identified plasma microRNA (miR) profiles that associate with markers of microvascular injury in patients with diabetic nephropathy (DN). However, miRs circulate in extracellular vesicles (EVs) or in association with HDL or the RNA-binding protein argonaute-2 (Ago-2). Given that the EV- and HDL-mediated miR transfer toward endothelial cells (ECs) regulates cellular quiescence and inflammation, we hypothesized that the distribution of miRs among carriers affects microvascular homeostasis in DN. Therefore, we determined the miR expression in EV, HDL, and Ago-2 fractions isolated from EDTA plasma of healthy control subjects, patients with diabetes mellitus (DM) with or without early DN (estimated glomerular filtration rate [eGFR...
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