Applications of natural deep eutectic solvents to extraction and preservation of biomolecules

The recently introduced nature-originated deep eutectic solvents, so-called natural deep eutectic solvents (NADES) are considered as truly green solvents, which composed of natural ingredients found abundantly in organisms. NADES has been used in diverse applications, including extraction of bioactive molecules, enzymatic processes, agricultural and pharmaceutical fields, and protein stabilization. Despite the various applications, their physiological roles in organisms still remain unclear. In the thesis, the possible roles of NADES were investigated using a model plant (Drosera species) and for macromolecules. The chemical analysis of Drosera exudates indicated that the exudates contained sugars, amino acids, and organic acids which might be NADES components, and they could be involved in the biological roles of exudates. Several NADES are also proven to be optimum media to improve the stability and activity of enzymes. Additionally, NADES protected the enzymes from microorganism contamination. Besides enzymes, DNA and RNA were well protected from degradation by NADES. Based on the results of the thesis, a new biological role of NADES associated to macromolecules were deduced.Plant science