The enzyme 5-lipoxygenase (5-LOX) initiates biosynthesis of the proinflammatory leukotriene lipid mediators and, together with 15-LOX, is also required for synthesis of the anti-inflammatory lipoxins. The catalytic activity of 5-LOX is regulated through multiple mechanisms, including Ca 2+-targeted membrane binding and phosphorylation at specific serine residues. To investigate the consequences of phosphorylation at S663, we mutated the residue to the phosphorylation mimic Asp, providing a homogenous preparation suitable for catalytic and structural studies. The S663D enzyme exhibits robust 15-LOX activity, as determined by spectrophotometric and HPLC analyses, with only traces of 5-LOX activity remaining; synthesis of the anti-inflammatory...
Resolution of acute inflammation is an active process coordinated by proresolving lipid mediators (S...
Leukotrienes are lipid messengers involved in autocrine and paracrine cellular signaling. They are s...
Leukotrienes (LT) are lipid mediators of the inflammatory response that are linked to asthma and ath...
The synthesis of both proinflammatory leukotrienes and anti-inflammatory lipoxins requires the enzym...
© 2018 Elsevier B.V. Leukotrienes (LT) are lipid mediators of the inflammatory response that play ke...
This work describes the structure of human 5-lipoxygenase (5-LOX) (Gilbert, Bartlett et al. 2011) an...
Leukotrienes are potent immune-modulatory compounds involved in the inflammatory response. 5-Lipoxyg...
Arachidonic acid (AA) is the precursor to leukotrienes (LT), potent mediators of the inflammatory re...
Leukotrienes are inflammatory mediators involved in several diseases. The enzyme 5-lipoxygenase init...
The synthesis of proinflammatory leukotrienes implicated in asthma, allergic rhinitis, and atheroscl...
AbstractLipoxygenases (LOXs) and cyclooxygenases (COXs) metabolize poly-unsaturated fatty acids into...
5-LOX is a non-heme iron dioxygenase that catalyzes the synthesis of a broad class of compounds, kno...
Lipoxins are an important class of lipid mediators that induce the resolution of inflammation and ar...
For disease network intervention, up-regulating enzyme activities is equally as important as down-re...
Lipoxins are an important class of lipid mediators that induce the resolution of inflammation and ar...
Resolution of acute inflammation is an active process coordinated by proresolving lipid mediators (S...
Leukotrienes are lipid messengers involved in autocrine and paracrine cellular signaling. They are s...
Leukotrienes (LT) are lipid mediators of the inflammatory response that are linked to asthma and ath...
The synthesis of both proinflammatory leukotrienes and anti-inflammatory lipoxins requires the enzym...
© 2018 Elsevier B.V. Leukotrienes (LT) are lipid mediators of the inflammatory response that play ke...
This work describes the structure of human 5-lipoxygenase (5-LOX) (Gilbert, Bartlett et al. 2011) an...
Leukotrienes are potent immune-modulatory compounds involved in the inflammatory response. 5-Lipoxyg...
Arachidonic acid (AA) is the precursor to leukotrienes (LT), potent mediators of the inflammatory re...
Leukotrienes are inflammatory mediators involved in several diseases. The enzyme 5-lipoxygenase init...
The synthesis of proinflammatory leukotrienes implicated in asthma, allergic rhinitis, and atheroscl...
AbstractLipoxygenases (LOXs) and cyclooxygenases (COXs) metabolize poly-unsaturated fatty acids into...
5-LOX is a non-heme iron dioxygenase that catalyzes the synthesis of a broad class of compounds, kno...
Lipoxins are an important class of lipid mediators that induce the resolution of inflammation and ar...
For disease network intervention, up-regulating enzyme activities is equally as important as down-re...
Lipoxins are an important class of lipid mediators that induce the resolution of inflammation and ar...
Resolution of acute inflammation is an active process coordinated by proresolving lipid mediators (S...
Leukotrienes are lipid messengers involved in autocrine and paracrine cellular signaling. They are s...
Leukotrienes (LT) are lipid mediators of the inflammatory response that are linked to asthma and ath...