Study of transport mediated by drug transporters in model cell systems

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Jana Znamínková Supervisor: PharmDr. Lucie Hyršová, PhD. Title of diploma thesis: Study of transport mediated by drug transporters in model cell systems Liver is the main biotransformation organ of human organism. Not every substance can cross the cell membrane of hepatocytes by simple diffusion. Transport of such substances across the membrane is mediated by specialized transport proteins. Uptake transporters localized on the basolateral membrane of hepatocytes play a crucial role in uptake of compounds from blood into hepatocytes, where the biotransformation takes place. OCT1 transporter belongs between important members of this group, it transports organic cations. Efflux transporters localized on the apical membrane are responsible for transport of substances from hepatocytes to bile canalicular and these transporters mediate their elimination in this way. BCRP is an example of this group of transporters. Drug transporters represent one of the most important mechanisms of drug-drug interactions due to their wide range of substrates. The aim of this study was to evaluate the function of novel cell models using substrate and inhibitors of these transporters. Stably transfected MDCK II cells..