The Effect of Renal Ischemia-Reperfusion Induced Oxidative Stress Damage on the Pancreas: Role of Dose-Dependent

The purpose of this studywas to demonstrate the effects of dosing-dependent boric acid (BA) administration on the renal I/R effect on remote tissue pancreas function. For this purpose, 35 Spraque Dawley albino rats were divided into five groups as 7 animals in each group: sham, I/R, I/R + 50 mg kg of BA, I/R + 100 mg/kg of BA and I/R + 200 mg/kg of BA. Sham group was only subjected to surgical stress procedure. In the I/R group, the left renal artery was isolated and ischemia was induced with antitravmatic vascular clamp for 45 minutes, followed by reperfusion for 24 hours. In the I/R+ BA groups, boric acid was administered intraperitoneally for 10 minutes before reperfusion. Blood urea nitrogen (BUN) and creatinine levels were measured to determine I/R damage in the kidney. Insulin, amylase and glucose levels were assessed for pancreatic functions as distant tissue damage. CAT, SOD, MDA and glutathione levels were recorded in order to show the oxidative stress damage and protective effects of boric acid on pancreatic tissue. In conclusion, this study demonstrated the protective effects of boric acid against remote tissue pancreatic injury caused by renal ischemia/reperfusion