THE EFFECTS OF OPTIMIZATION METHODS ON THE DETERMINATION OF TOTAL ANTIOXIDANT CAPACITY IN SOME PLANTS

Phytochemical properties and prophylaxis of medicinal plants in alternative medicine applications have been emphasized in many studies. Antioxidants are effective particularly in terminating chain reactions, which are caused by free radicals formed during metabolic activity reactions. The elution of these components is usually carried out with solvent-extraction procedure. In this study, it was aimed to measure total antioxidant capacities (TAC) of Equisetum arvense and Lycopodium clavatum plant species that grown in Turkey, using different methods with infusion, boiling and methanol extracts. It was observed that TAC of the extracts prepared by the magnetic stirrer obtained higher yields compared to other methods but there was no statistically significant difference. In temperature studies, the most efficient results for TAC values were determined at 100 degrees C degrees and statistically significant differences were determined (p<0.001). It was observed that the amount of TAC decreased statistically after 10 minutes in the optimum time determination study (p