Addition of Coconut Water and Banana Extract on MS Media to Stimulate PLB (Protocorm Like Bodies) Regeneration of Dendrobium gatton sunray

Organic material is widely used for propagation of orchids, namely coconut water, extracts of tomatoes, bananas, bean sprouts and potatoes which can be added to certain media as a provider of nutrients, amino acid minerals, growth regulators. This study aims to determine the effect of the concentration of organic matter in coconut water and banana extract on MS media on the regeneration of PLB (protocorn like bodies) Dendrobium gatton sunray. The study was conducted at the Biotechnology Laboratory, Faculty of Agriculture, Bengkulu University, from September to December 2019. This study used a Complete Randomized Block Design (RCBD) with two factors. The first factor was the concentration of coconut water with 3 levels namely: A0 = 0 (Control), A1 = 75 ml/l, A2 = 150 ml/l, and the second factor was the concentration of banana extract with 5 levels namely: B0 = 0 (Control), B1 = 75 g/l, B2 = 150 g/l. The results showed that the addition of coconut water to 75 ml/l on MS media increased the number of leaves, shoot length, number of roots, root length and plant weight. Banana extract 75 g/l on MS medium increased the number of shoots, the number of leaves and the number of roots. Overall, this study shows that the addition of organic coconut water and banana extract can be used to stimulate regeneration of PLB, which also functions as a natural growth regulator and a source of organic matter