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Not AvailableDrought stress is the major production constraint in rainfed maize. Screening for drought tolerance is severely affected by the lack of a simple and reliable phenotyping technique. The objective of this study was to standardize a simple hydroponic based drought screening technique in maize. In this context, one week old uniform seedlings of 55 inbreds and 5 hybrids were transferred to hydroponic solution in the glass house. The seedlings were allowed to acclimatize for next one week in hydroponic solution. The drought stress was imposed by removing seedlings from nutrient solution and exposed to air for 6 and 4 hours daily for a period of 5 and 4 consecutive days in hybrids and inbreds, respectively. Data were recorded on all shoot and root parameters, and based on stress symptoms, a drought tolerance score was given to each genotype. The percent deductions in shoot and root fresh weight from non-stress to stress ranged from 11.7 to 84.4 and 2.1 to 77.5, respectively. Six inbred lines, namely, DQL790-4, CML334, CM140, CML422, CM125 and HKI488 and three hybrids namely DMRH1306, DMRH1410 and PMH4 were found drought tolerant. The effectiveness of this screening technique was compared and confirmed using pots screening as well as by expression profiling of key antioxidant genes (Sod2, Sod4, Sod9 and Apx1) playing role in drought stress tolerance. This phenotyping technique is very short, low cost and simple which can be utilized in preliminary drought screening for large set of maize germplasm and mapping populations.Not Availabl