Design, Synthesis and Biological Evaluation of Pyrazoline Substituted Benzimidazole Derivatives as Anthelmintic Agents

Background: Benzimidazole nucleus is present in a number of pharmacologically active molecules such as Albendazole, Mebendazole (anthelmintic), omeprazole (anti-ulcer) and droperidol, pimozide (Anti-psychotic). Several 1,3,5-trisubstituted pyrazolines were found to possess anthelmintic activity. Aim: The present research focused on incorporating pyrazoline nucleus into benzimidazole nucleus to form a potent anthelmintic agent. Method: The in silico design of novel pyrazoline substituted benzimidazole derivatives was done using PASS software and the compounds found to be active in in silico studies are synthesized and in vitro screening of the compounds done for anthelmintic activities . Results: Anthelmintic activity studies of the proposed compounds done on earth worm (Eudrilus Eugenia) using Albendazole as standard. Benzimidazole derivative (BZ-6) found be the best anthelmintic agent in in vitro studies. Conclusion: Pyrazoline substituted Benzimidazole derivatives are potent anthelmintic agents. The proposed compounds are potential lead compounds for the development of a better anthelmintic drug. Further studies for toxicity prediction and in vivo studies have to be conducted