Comparing aspirations: intercalibration of ecological status concepts across European lakes for littoral diatoms.

Eleven countries participated in an intercalibration exercise to harmonise diatom-based methods used for status assessment in lakes. Participating countries extended from Sweden and Finland in the north to Italy and Slovenia in the south, and from Ireland in the west to Hungary in the east. Intercalibration followed standard procedures developed for the European Commission which ensures that outcomes of this exercise are compatible with those for other types of biota in both freshwater and marine water bodies throughout Europe. Lakes were divided into low, medium and high alkalinity types for this exercise. However, it was not possible to perform a full intercalibration on the low alkalinity lakes due to the short gradient length and confounding influences of pH and humic substances. Values of the Trophie Index of Rott et al. (1999) were computed for all samples in order that national datasets could all be expressed on a common scale. Not all participating countries had reference sites against which national methods could be standardised and, therefore, a Generalised Linear Modelling approach was used to control the effect of national differences in datasets. These steps enabled the position of high/good and good/moderate status boundaries for all participating countries to be expressed on a common scale and for deviations beyond 0.25 class widths ( 0.05 Ecological Quality Radio (EQR) units, assuming equal distances between all class boundaries) to be identified. Those countries which had relaxed boundaries were required to adjust these to within 0.25 class widths whilst the intercalibration rules allowed those countries with more stringent boundaries to retain these. Despite biogeographical and typological differences between participating countries, there was broad agreement on the characteristics of high, good and moderate status diatom assemblages, and the intercalibration exercise has ensured a consistent application of Water Framework Directive assessments around Europe.JRC.H.1-Water Resource