Direct Determination of Kinetic Rates from Single-Molecule Photon Arrival Trajectories Using Hidden Markov Models

The kinetic rates of single-molecule photon trajectories were determined using hidden Markov model (HMM). The data from single-molecule experiments were in the form of photon trajectories consisting of arrival times and wavelength information on individual photons. HMM was used as an alternative to higher-order correlation function analysis for the detection of conformational memory