A human HEK-293 cell line producing a recombinant interferon was further engineered to express a yeast pyruvate carboxylase (PYC) gene. In batch culture, the transformed cells exhibited marked reductions in both nutrient consumption and toxic metabolites formation. This altered metabolism led to a 2-fold increase in the maximum cell density and a 25 % increase in recombinant protein production. To further characterize the underlying metabolic changes of the PYC-expressing cells, we have conducted isotopic tracer studies using various singly and uniformly labeled 13C tracers. The resulting mass distributions of extracellular lactate determined by LC/MS analysis were used as inputs to a comprehensive metabolic model to calculate the intracell...
Chinese hamster ovary (CHO) cells are the dominant host for producing biotherapeutic proteins. Despi...
Animal cell culture processes are needed for the production of monoclonal antibodies, recombinant pr...
The production of virus-like particles (VLPs) has gained importance over the last few years owing to...
HEK-293 is the most extensively used human cell line for the production of viral vectors and is gain...
The accumulation of metabolic by-products remains a critical challenge in the development of mammali...
AbstractRecombinant yeast pyruvate carboxylase (PYC2) expression was previously shown to be an effec...
<div><p>Chinese hamster ovary (CHO) cells are the most preferred mammalian host used for the bio-pha...
Chinese hamster ovary (CHO) cells are the most preferred mammalian host used for the bio-pharmaceuti...
Hek293 cells are the predominant hosts for transient expression of recombinant proteins and are used...
Hek293 cells are the predominant hosts for transient expression of recombinant proteins and are used...
Niklas J, Skerhutt EM, Sandig V, Noll T, Heinzle E. Quantitative characterization of metabolism and ...
<div><p>Hek293 cells are the predominant hosts for transient expression of recombinant proteins and ...
ABSTRACT: HEK293 cells are gaining in interest for the production of recombinant proteins. However, ...
Industrial bioprocesses place high demands on the intermediary metabolism of host cells to meet the ...
ABSTRACT: Recombinant yeast pyruvate carboxylase (PYC2) expression was previously shown to be an eff...
Chinese hamster ovary (CHO) cells are the dominant host for producing biotherapeutic proteins. Despi...
Animal cell culture processes are needed for the production of monoclonal antibodies, recombinant pr...
The production of virus-like particles (VLPs) has gained importance over the last few years owing to...
HEK-293 is the most extensively used human cell line for the production of viral vectors and is gain...
The accumulation of metabolic by-products remains a critical challenge in the development of mammali...
AbstractRecombinant yeast pyruvate carboxylase (PYC2) expression was previously shown to be an effec...
<div><p>Chinese hamster ovary (CHO) cells are the most preferred mammalian host used for the bio-pha...
Chinese hamster ovary (CHO) cells are the most preferred mammalian host used for the bio-pharmaceuti...
Hek293 cells are the predominant hosts for transient expression of recombinant proteins and are used...
Hek293 cells are the predominant hosts for transient expression of recombinant proteins and are used...
Niklas J, Skerhutt EM, Sandig V, Noll T, Heinzle E. Quantitative characterization of metabolism and ...
<div><p>Hek293 cells are the predominant hosts for transient expression of recombinant proteins and ...
ABSTRACT: HEK293 cells are gaining in interest for the production of recombinant proteins. However, ...
Industrial bioprocesses place high demands on the intermediary metabolism of host cells to meet the ...
ABSTRACT: Recombinant yeast pyruvate carboxylase (PYC2) expression was previously shown to be an eff...
Chinese hamster ovary (CHO) cells are the dominant host for producing biotherapeutic proteins. Despi...
Animal cell culture processes are needed for the production of monoclonal antibodies, recombinant pr...
The production of virus-like particles (VLPs) has gained importance over the last few years owing to...