Microencapsulated mammalian cells for simultaneous production of VEGF165b and IFN\u3b1

Targeted and simultaneous delivery VEGF165b and IFN alpha in anti-angiogenic and other applications could offer several advantages. For this a system was design using artificial cell alginate-poly-L-lysine- alginate (APA) microcapsules. Result confirms the ability of this system for simultaneous production of these proteins for 28-days. The IFN alpha on a 3 days period increased from 8 \ub1 0.36 \u3bcg/ml at day 10 to 27 \ub1 2.4 \u3bcg/ml at day 16 and then dropped to 6.5 \ub1 0.5 \u3bcg/ml. The VEGF165b on a 3 days period increased from 2.7 \ub1 0.7 \u3bcg/ml at day 10 to 6.9 \ub1 1 \u3bcg/ml at day 16.Peer reviewed: YesNRC publication: Ye