Studies on Marine Bacteria Producing Lytic Enzymes-Ⅴ Culture Condition for the Production of Lytic Enzyme

The production of lytic enzyme by strain V 37 was investigated using polypeptone-yeast extract medium or chemically defined medium under various culture conditions. Strain V 37 grew well and also produced lytic enzyme at pH 5.0, 6.0, 7.0 and 8.0 in polypeptone-yeast extract medium containing 0.5 mole /l of NaCl. The high yield of lytic enzyme was observed in the media containing NaNO3 （0.1-1.0 mole/l) or NaSCN (0.2-0.3 mole/l). Chloride (Cl-) and nitrate (NO3−）ions were more effective than thiocyanate (SCN-), dihydrogenphosphate (H2PO4-) and sulfate (SO42-) ions for the production of lytic enzyme. The elevated MgCl2 in the presence of NaCl rather inhibited the production of lvtic enzyme. Strain V 37 could not grow in a chemically defined medium proposed by MACLEOD (1968) during 24 hours of cultivation