The role of whole genome sequencing in characterizing the mechanism of action of para-aminosalicylic acid and its resistance

Para-aminosalicylic acid (PAS) remains one of the drugs of last resort for the treatment of tuberculosis, but its mechanism of action is not completely understood yet. The main aim of this project was to identify new potential mechanisms of action and resistance to PAS by performing whole genome sequencing (WGS) on PAS-resistant laboratory mutants. A new variant in the folC gene has been identified as well as some other mutations that require further studies