The function of initiation factors in and the sequence of events during translation initiation have been intensively studied in Bacteria and Eukaryotes, whereas in Archaea knowledge on these functions/processes is limited. By employing chemical probing, we show that translation initiation factor aIF1 of the model crenarchaeon Sulfolobus solfataricus binds to the same area on the ribosome as the bacterial and eukaryal orthologs. Fluorescence energy transfer assays (FRET) showed that aIF1, like its eukaryotic and bacterial orthologs, has a fidelity function in translation initiation complex formation, and that both aIF1 and aIF1A exert a synergistic effect in stimulating ribosomal association of the Met-tRNAi(Met) binding factor a/eIF2. Howev...
International audienceThe heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal i...
International audienceTo carry initiator Met-tRNA(i)(Met) to the small ribosomal subunit, eukaryal a...
International audienceTranslation initiation (TI) allows accurate selection of the initiation codon ...
The function of initiation factors in and the sequence of events during translation initiation have ...
International audienceIn eukaryotes and in archaea late steps of translation initiation involve the ...
The eukaryotic translation initiation factor 1 binds to the ribosome during translation initiation. ...
The eukaryotic translation initiation factor 2 (eIF2) is pivotal for delivery of the initiator tRNA ...
International audienceInitiation of translation in eukaryotes and in archaea involves eukaryotic/arc...
International audienceEukaryotic and archaeal initiation factors 2 (e/aIF2) are heterotrimeric prote...
Initiation is a critical step in translation, during which the ribosome lands on the start codon and...
International audienceA critical consequence of the initiation of translation is the setting of the ...
Translation is an important step in gene expression. Initiation of translation is rate-limiting, and...
The formation of the translation initiation complex represents the rate-limiting step in protein syn...
International audienceThe heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal i...
International audienceTo carry initiator Met-tRNA(i)(Met) to the small ribosomal subunit, eukaryal a...
International audienceTranslation initiation (TI) allows accurate selection of the initiation codon ...
The function of initiation factors in and the sequence of events during translation initiation have ...
International audienceIn eukaryotes and in archaea late steps of translation initiation involve the ...
The eukaryotic translation initiation factor 1 binds to the ribosome during translation initiation. ...
The eukaryotic translation initiation factor 2 (eIF2) is pivotal for delivery of the initiator tRNA ...
International audienceInitiation of translation in eukaryotes and in archaea involves eukaryotic/arc...
International audienceEukaryotic and archaeal initiation factors 2 (e/aIF2) are heterotrimeric prote...
Initiation is a critical step in translation, during which the ribosome lands on the start codon and...
International audienceA critical consequence of the initiation of translation is the setting of the ...
Translation is an important step in gene expression. Initiation of translation is rate-limiting, and...
The formation of the translation initiation complex represents the rate-limiting step in protein syn...
International audienceThe heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal i...
International audienceTo carry initiator Met-tRNA(i)(Met) to the small ribosomal subunit, eukaryal a...
International audienceTranslation initiation (TI) allows accurate selection of the initiation codon ...