Plasmolysis: Loss of Turgor and Beyond -- dataset

Movie S1: Distribution and motility of GFP-MAP4 in plasmolysis and deplasmolysis (0.8 M mannitol), overlay of fluorescent and bright field images. Time lapse movie of the Arabidopsis hypocotyl cells shown in Figure 3. Movie acquired over 20 min, images acquired at 2 min intervals. Movie S2: Fluorescent images of a GFP-ABD line of Arabidopsis showing endoplasmic actin and cortical actin in hypocotyl cells. Actin is visible in the cortex of the protoplast in plasmolysis and also in deplasmolysis. Hechtian strands are incorporated when the protoplast expands in deplasmolysis. Movie acquired before plasmolysis, after 30 min of plasmolysis and over 20 min during deplasmolysis, all images acquired at 2 min intervals. Movie S3: Evidence for dynamics of cytoplasmic organelles in bright field mode during plasmolysis and expansion of the cells in deplasmolysis. Images acquired every min, over a period of 20 min