Essential role of adiponectin in ischemic postconditioning cardioprotection and its association with signal transducer and activator of transcription 3

Conference Theme: Transforming the Future through SciencePoster Session: Pharmacology and Experimental Therapeutics - Cell Injury/Cell DeathIschemic postconditioning (IPostC) protects the heart against ischemia reperfusion injury (IRI) by activating signal transducer and activator of transcription 3 (STAT3). Adiponectin (APN), a protein with anti-IRI property, activates STAT3 (Cell Metab. 2011, 13: 401-12), but its role in IPostC is unknown. We postulated that APN is essential for IPostC to confer cardioprotection. Wild type (WT) and APN knockout (KO) mice were subjected to 30 min coronary occlusion followed by 120 min reperfusion without or with IPostC achieved by 3 cycles of 10s of ischemia/10s of reperfusion. Cardiac function was assessed by pressure-volume (PV) loop system. At the end of reperfusion, KO mice displayed larger infarct size and higher plasma creatine kinase-MB, but lower end-systolic PV relation (a reliable measure of ventricular function) and dP/dt (all P