Determination of phenols in water by high performance liquid chromatography with a UV-Detector

This study is based on the SPE process, and the method employed was validated on 11 compounds of phenol using phenol calibration mix. It focuses on the determination of phenols in water by high performance liquid chromatography with a UV-detector. The background section deals with phenols, its subsequent group, the analytical methods and laboratory equipments used in determining these compounds in water. The experimental section provides information on the validation and optimization of analytical method according to the ISO standard or EPA method concentrates on specific group of phenols. High performance liquid chromatography with a UV-detector is the main analytical equipment used in determination of phenols in water. The water samples to be analyzed were taken from five different locations in Poznan, Poland. The experiments were done in the water and soil testing laboratory of regional sanitary and epidemiological station in Poznan, Poland. The objective of the developed method was to achieve a concentration of 0.1 mg/l of each phenol from water samples and excellent recovery between 80-110% based on the column employed. In conclusion, with the method developed, it can be prove that phenols were not present in water within the working range of 0.003-0.250 mg/l