Studies on lectin : I Survey of Okinawan leguminosae seeds and other plants for hemagglutination and purification of Glycine max lectin cultivated in Okinawa islands (Department of Agricultural Chemistry)

沖縄産豆科植物種子およびその他植物の生理食塩水抽出液によるウサギ赤血球凝集活性の検索および大豆レクチンの精製を試みた。未処理およびトリプシン処理赤血球に対し凝集活性を示す種子は, Canavalia gladiataおよびmicrocarpa, Dolichos lablab var 1および2,Erythria variegata, Galactia tashiroi, Glycine maxおよびPsophocarpus tetragonolobusの8種であった。一方, 豆科植物以外ではAlocasia odora抽出液によりトリプシン処理赤血球は凝集した。Glycine max(沖縄産大豆)レクチンの血液型および単糖に対する特異性を調べた結果, 本レクチンはヒト血液型に対し非特異的であり, ガラクトース型レクチンであった。硫酸アンモニウムによる部分分画およびハイドロキシルアパタイトカラムクロマトグラフィーにより, CM-セルロースおよび電気泳動的に均一な精製レクチンが得られた。Survery of extracts of Okinawan leguminosae seeds and other plants for hemagglutination and purification of Glycine max lectin were studied. Leguminosae family seeds showing hemagglutinating activity for non- and trypsin-treated rabbit red blood cell (RBC) were as follows; Canavalia gladiate and microcarpa, Dolichos lablab var. 1 and var. 2,Erythria variegata, Galactia tashiroi, Clycine max and Psophocarpus tetragonolobus. While, Alocasia odora extracts which belongs to family of Araceae agglutinated trypsin-treated RBC. The specificity of the crude G. max lectin was tested by hemagglutination-inhibition and human ABO types assays and was found to be galactose specific lectin and non-specific toward ABO types. The purification of G. max lectin was accomplished first by fractional precipitation with ammonium sulfate, and then by hydroxylapatite column chromatography. Proteins so obtained revealed to be homogenous by CM-cellulose column chromatography and disk electrophoresis