Efek Pemberian Kombinasi Ekstrak Meniran (Phyllanthus niruri Linn.) Dan Sambiloto (Andrographis paniculata Nees.) Terhadap Luas Germinal Center Limpa Ayam Layer Yang Diinfeksi APEC (Avian Pathogenic Escherichia coli)

The aim of this research was to know the effect of combination meniran extract (Phyllanthus niruri Linn.) and sambiloto extract (Andrographis paniculata Nees.) to the germinal center area of spleen laying hens infected by APEC (Avian Pathogenic Escherichia coli). This research used twenty-four of thirty-two weeks old layer hens, divided into six treatment groups, each group contain four-layer hens. Treatment groups consists of P0(-) (suspension of CMC Na 0.5% + 0.9% saline solution); P0(+) (Suspension of CMC Na 0.5% + APEC bacteria with concentration 108 CFU/ml); P0(Extract) (combination of meniran extract with 20% concentration and sambiloto extract with 20% concentration + 0.9% saline solution); P1, P2, and P3 (each of group given with meniran extract and sambiloto extract 30% and 10%; 20% and 20%; 10% and 30% + APEC bacteria with 108 CFU/ml). Observation with measurement of the germinal center area. Data analysis was done with the ANOVA test and Duncan post hoc. This research showed a significant difference (p< 0.05) between each treatment group. The conclusion of this research showed that combination meniran extract concentration of 20% and sambiloto extract concentration of 20% as a could increase the germinal center area of layer hens spleen to the highest value