Pengaruh Vanadil Sulfat Terhadap Aktivitas Glucose Trasporter 4 Jaringan Otot dan Adiposa Mencit (Mus Musculus) yang Menderita Diabetes Mellitus

The present study was designed to investigate the effects of vanadyl sulphate (VS) toward both hystochemistry and immunohystochemistry - related to glucose transporter 4 (GLUT4) activity - alteration of skeletal muscle and adipose tissue in diabetic mice. Forty mice were divided into five groups, which were positive control (normal) group, negative control (diabetic) group, and three groups differed by vanadyl sulphates doses (5 mg/kg BW, 30 mg/kg BW, or 100 mg/kg BW). Mice were made in diabetic condition by two stretozotocin (STZ) inductions. The first induction, 100 mg/kg BW, was given on the first day. While the second, 50 mg/kg BW, was given on the 14th day. Diabetic condition occured on day 21 after the first induction, shown by increasing blood glucose level from (101.00 ± 9.19) mg/dL to (155.60 ± 18.84) mg/dL. Treatment of VS was given on the 21th day. Administrations of vanadyl sulphate in several doses were significantly reduced blood glucose concentration [F(4,16) = 13.716; p < 0.001]. On the day 28, skeletal muscle and adipose tissue were harvested and checked hystochemically by haematoxyllin-eosin (HE) staining. The results of hystochemistry showed that VS can improve an atrophy and necrosis in muscle. In adipose tissue, VS inhibits lipolysis and restores the morphology of adipocyte cells. While the results of immunohystochemistry showed that VS can increase GLUT4 translocation from cytoplasm to the surface membrane of muscle cell, therefore the activity of this protein is rising. Keywords: vanadyl sulphate, STZ, DM, GLUT4, immunohistochemistry, muscle, adipos