Effect of Incubation Time on Protein Loading and Encapsulation Efficiency of Ovalbumin-Alginate Microspheres

The aim of this research was to investigate effect of incubation time that was important part on determination protein content method toward encapsulation efficiency and protein loading of microspheres. Determination method ofencapsulation efflciency and protein loading used on this research by addition ofsodium citrate solution 0.5 M pH 8.5 for 3,6, g, and 12 hours. Results showed that encapsulation efficiency and protein loading of ovalbumin-Alginate microspheres after 3, 6, 9 and 12 hours incubation were above 99oh and above 38% respectively. Based on statistical analysis, it was found that no significant differences between encapsulation efficiency and protein loading at different incubation times. Results of size before incubation was 5.87pm and after 3, 6, 9 and 12 hours incubation was 2.45pm 2.38ptm, 2.39pm, and 2.42pm respectively. Different incubation times produced similar size of particle; and same high encapsulation efficiency and protein loading as size. In addition, Biuret test demonstrated that protein was still existed after several hours' incubation