PENGARUH KADAR HPMC K4M TERHADAP VIABILITAS DAN DAYA PROTEKSI MIKROPARTIKEL Lactobacillus casei FNCC 0090 PADA ASAM DENGAN MATRIKS KOPOLIMER ASAM METAKRILAT TIPE L

Probiotics are living microorganisms that can provide health benefits on 106-107 cfu/gram. Lactobacillus casei is one of probiotic that unstable in gastric acid despite having intestinal target of action. The delivery system that can be used to deliver Lactobacillus casei to its target site in the intestine is a microparticle that can be done by spray drying method and following pulsatile release. This research was investigate the effect of 0.0%, 0.2%, 0.3%, and 0.4% HPMC K4M content on viability and gastric resistant of Lactobacillus casei in microparticles with 1% L-type methacrylic acid copolymer matrix after spray drying process. The morphology of microparticle in four formulas was described as follows: formula I had a spherical form with shallow basin. Formula II, III, and IV with HPMC K4M content level of 0.2%, 0.3%, and 0.4% respectively, known to be less spherical and had a deep-seated, shrunken crease. The percentage of bacterial viability of the formula I, II, III, and IV ± standard deviation were 78.95 ± 4.45, 77.54 ± 4.36, 79.45 ± 1.14, and 89.66 ± 3.86 respectively, which showed a percentage increase in viability of Lactobacillus casei significantly. Gastric resistant test results indicated that the percentage of acid protection from each of the formula decreased from formulas I, II, III, and IV were 63.85± 8.21, 66.75±14.13, 63.53±12.02, dan 59.82±14.63 as HPMC levels increase. Statistically, it showed no significant difference from any formula. The research conclude that microparticle Lactobacillus casei with L-type methacrylic acid copolymer matrix shows good viability and gastric resistant for intestinal targeted site drug delivery system