PENGARUH BEVACIZUMAB TERHADAP EKSPRESI KOLAGEN TIPE I PADA AREA TRABEKULEKTOMI (Studi Eksperimental pada Oryctolagus cuniculus)

Objective : to determine the effect of bevacizumab as an antifibrotic agent on collagen density, collagen thickness and collagen type I after trabeculectomy in rabbit. Materials and methods : Sixteen male New Zealand white rabbits divided by two groups, 8 rabbits in control group and 8 rabbits in treatment group. Control group underwent trabeculectomy and injection of balanced salt solution. Treatment group underwent trabeculectomy and subconjunctival injection of bevacizumab (1.25mg, 25mg/mL). They were terminated on 14 postoperative days. Masson Trichrome were performed to evaluate collagen collagen density, collagen thickness. Immunohistochemistry using a monoclonal antibody to collagen type I was performed to evaluate collagen type I expression. Results : This study showed the density of collagen fibers decreased and statistically significant in the treatment group (p = 0.075, p <0.05), whereas the the thickness of collagen fibers did not have a significant decrease in both groups (p = 0.323, p > 0.05). Expression of type I collagen obtained a decrease in the treatment group compared to BSS group (p = 0.006, p