UJI AKTIVITAS ANALGESIK SENYAWA 4-NITROBENZOIL-3-ALILTIOUREA PADA MENCIT (Mus musculus) DENGAN METODE WRITHING TEST

Synthesis of 4-nitrobenzoyl-3-allylthiourea was accomplished by acylated allylthiourea with 4-nitrobenzoyl chloride. The acylation procedure based on Schotten-Baumann reaction modification triethylamine as a base and a catalyst. The percent yield of the product of synthesis was 28%. The purity of the compound was analyzed by Thin Layer Chromatography and melting point test. According to the UV-Vis and FT-IR spectra analysis, it showed that 4-nitrobenzoyl-3-allylthiourea was successfully synthesized. Analgesic activity of the compound was tested in mice (Mus musculus) using writhing test method with 0,6% acetic acid as the pain inducer. The doses of the 4-nitrobenzoyl-3-allylthiourea are 10 mg/KgBW, 20 mg/kgBW, and 40 mg/KgBW was given in mice by intraperitoneal injection. The result of ED50 for 4-nitrobenzoyl-3-allylthiourea is 12,5 mg/Kg body weight, for allylthiourea is 21,87 mg/Kg body weight, and for diclofenac natrium is 14,79 mg/Kg body weight. Statistically, the analgesic activity of 4-nitrobenzoyl-3-allylthiourea was higher compare with allylthiourea and diclofenac natrium