UJI PELEPASAN TRETINOIN SEBAGAI ANTIAGING DALAM SISTEM EMULSI KONVENSIONAL DAN NANOEMULSI MENGGUNAKAN VIRGIN COCONUT OIL

The aim of this study was to comparised release rate of tretinoin from conventional emulsion and nanoemulsion vehicle using virgin coconut oil as oil phase; Span 80 & Tween 80 as surfactants – 96% ethanol as cosurfactant; and a solution of phosphate buffer pH 6,0±0,5 as water phase (with ratio 1 : 9 : 27,5). Drug release was determined using Franz Diffusion cell with cellophane were used as a membrane and mixed solvent (methanol:phosphate buffer pH 6,0±0,5 = 2:1) as receptor solution for 12 hours. The temperature was controlled at 32ºC ± 0,5ºC. The result of this study were 0,0410 μg/cm2.min for nanoemulsion and 0,0413 μg/cm2.min for emulsion, then divided into several stages of time. Early stage was in minutes to 0 up to 5, both of vehicles were released tretinoin with nearly same amount. Next stage was in minutes to 5 up to 60, showed that drug release rate (flux) tretinoin from nanoemulsion higher than emulsion were 0,158 ±0.016 and 0,048 ±0.016 μg/cm2.min. Next stages was to 60 to 180 minutes, nanoemulsion showed drug release rate lower than emulsion were 0,046 ± 0.005 and 0,090 ± 0.016 μg/cm2.min. In minutes 180 to 720, both of nanoemulsion and emulsion were had nearly drug release rate were 0,025±0.001 and 0,022±0.002 μg/cm2.min. Results was statistically using independent sample t-test with degree of confident 95% (α=0,05). There were significant differences of tretinoin release rate. Keywords : Tretinoin, virgin coconut oil, nanoemulsion, conventional emulsion, drug releas