PENENTUAN PROFIL PROTEIN BIOFILM Streptococcus mutans UNTUK EKSPLORASI BIOMARKER RISIKO KARIES GIGI

Biofilm consist of a collection of microorganisme witch attached to a surface and is covered by adhesive polysaccharides had excreted by these microorganisme. One of the negative influence of biofilm on human life is the emergence of dental caries. Streptococcus mutans is a virulent bacteria to biofilm formation causes dental caries. Dental caries can be several disease, including mediastinitis, sepsis, facial cellulitis, osteomyelitis, endocarditis, and pneumonia. Caries index in Indonesia around 2.2, caries index WHO’s target is 1.0. Alternative effort to suppress dental caries with early diagnostic of dental caries risk using biomarker. Biofilm growth performed of S. mutans on glass slide to BHIBS media for 24 hours. Crude protein extract of biofilm had obtained by lysis using ultrasonication 7 x 30s, 40 Hz. Furthermore, analysis antigenic protein biofilm S. mutans using Western blot methode. In this study also have done the determination protein profiles of S. mutans biofilm using SDS-PAGE 12%. Protein profile of S. mutans biofilm consist of seventeen bands, with known size 190, 185, 175, 157, 105, 85, 66, 55, 52, 42, 40, 29, 25, 22, 20,18, and 17 kDa. Antigenic protein of S. mutans biofilm consist of four bands, with known size 105, 52, 40, and 29 kDa