Effects of silver diamine fluoride on dentine carious lesions induced by Streptococcus mutans and Actinomyces naeslundii biofilms

Background. Silver diamine fluoride (SDF) has been shown to be a successful treatment for arresting caries. However, the mechanism of SDF is to be elucidated. Aim. To characterize the effects of SDF on dentine carious induced by Streptococcus mutans and Actinomyces naeslundii. Design. Thirty-two artificially demineralized human dentine blocks were inoculated: 16 with S. mutans and 16 with A. naeslundii. Either SDF or water was applied to eight blocks in each group. Biofilm morphology, microbial kinetics and viability were evaluated by scanning electron microscopy, colony forming units, and confocal microscopy. The crosssection of the dentine carious lesions were assessed by microhardness testing, scanning electron microscopy with energy-dispersive x-ray spectroscopy and Fourier transform infrared spectroscopy. Results. Biofilm counts were reduced in SDF group than control (P<0.01). Surfaces of carious lesions were harder after SDF application than after water application (P<0.05), in S. mutans group, Ca and P weight percentage after SDF application than after water application (P<0.05). Lesions showed a significantly reduced level of matrix to phosphate after SDF treatment (P