Increase in interstitial ATP of rat soleus muscle during lactic acid infusion

Ballard, HJ
Tu, J

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Publication date

January 2007

Publisher

Federation of American Societies for Experimental Biology.

Journal

The FASEB Journal

Abstract

We investigated the effect of acidosis on ATP efflux from muscle. Lactic acid (LA) was infused into the arterial supply of the buffer-perfused rat hindlimb muscle; ATP concentrations were measured by HPLC in interstitial microdialysate from soleus muscle. Inhibitors of several ATP transporters were used to explore the mechanism. LA (10 mM) reduced venous pH from 7.14±0.02 to 7.00±0.03 (P<0.01) and increased interstitial ATP from 31±6 nM to 62±10 nM (P<0.01, n=13). Infusion of alpha-cyano-4-hydroxycinnamic acid, a lactate-transporter-inhibitor, prevented the LA-infusion-induced increase in interstitial ATP, suggesting that cellular uptake of the LA is necessary for the stimulation of ATP efflux. LA-induced elevation of interstitial ATP was r...

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Increase in interstitial ATP of rat soleus muscle during lactic acid infusion

Abstract

Extracted data

Increase in interstitial ATP of rat soleus muscle during lactic acid infusion

Abstract

Extracted data

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