We demonstrate that it is possible to observe single fluorescent molecules using a standard fluorescence microscope with mercury lamp excitation and an inexpensive cooled charge-coupled device (CCD) camera. With this equipment, we have been able to observe single molecules of tetramethyl-rhodamine, rhodamine 6G, fluorescein isothiocyanate and green fluorescent protein. Immobilized molecules were observed both in air and in aqueous solution
Relatively simple modifications of an ordinary epifluorescence microscope have greatly reduced its b...
Tinnefeld P, Sauer M. Branching out of single-molecule fluorescence spectroscopy: challenges for che...
One of the more popular single-molecule approaches in biological science is single-molecule fluoresc...
We demonstrate that it is possible to observe single fluorescent molecules using a standard fluoresc...
In recent years, single-molecule and single-particle fluorescence microscopy has emerged as a tool t...
Signals from single fluorescent dye molecules were first measured in non-biological environments. So...
In recent years, single-molecule and single-particle fluorescence microscopy has emerged as a tool t...
In recent years, single-molecule and single-particle fluorescence microscopy has emerged as a tool t...
In recent years, single-molecule and single-particle fluorescence microscopy has emerged as a tool t...
Two decades ago it was commonly believed that single molecules were too small to be seen using an op...
AbstractSingle-molecule fluorescence microscopy is a powerful tool for observing biomolecular intera...
An apparatus for single-molecule imaging was constructed and used to examine nanomolar solutions of ...
AbstractVisualization and tracking of single fluorescent molecules is a recent development in optica...
One of the more popular single-molecule approaches in biological science is single-molecule fluoresc...
An apparatus for single-molecule imaging was constructed and used to examine nanomolar solutions of ...
Relatively simple modifications of an ordinary epifluorescence microscope have greatly reduced its b...
Tinnefeld P, Sauer M. Branching out of single-molecule fluorescence spectroscopy: challenges for che...
One of the more popular single-molecule approaches in biological science is single-molecule fluoresc...
We demonstrate that it is possible to observe single fluorescent molecules using a standard fluoresc...
In recent years, single-molecule and single-particle fluorescence microscopy has emerged as a tool t...
Signals from single fluorescent dye molecules were first measured in non-biological environments. So...
In recent years, single-molecule and single-particle fluorescence microscopy has emerged as a tool t...
In recent years, single-molecule and single-particle fluorescence microscopy has emerged as a tool t...
In recent years, single-molecule and single-particle fluorescence microscopy has emerged as a tool t...
Two decades ago it was commonly believed that single molecules were too small to be seen using an op...
AbstractSingle-molecule fluorescence microscopy is a powerful tool for observing biomolecular intera...
An apparatus for single-molecule imaging was constructed and used to examine nanomolar solutions of ...
AbstractVisualization and tracking of single fluorescent molecules is a recent development in optica...
One of the more popular single-molecule approaches in biological science is single-molecule fluoresc...
An apparatus for single-molecule imaging was constructed and used to examine nanomolar solutions of ...
Relatively simple modifications of an ordinary epifluorescence microscope have greatly reduced its b...
Tinnefeld P, Sauer M. Branching out of single-molecule fluorescence spectroscopy: challenges for che...
One of the more popular single-molecule approaches in biological science is single-molecule fluoresc...