We recently designed a sequence-specific DNA-cleaving metalloprotein consisting wholly of naturally occurring α-amino acids. The tripeptide copper-binding domain, Gly-Gly-His (GGH), was attached to the NH2-terminus of the DNA-binding domain of Hin recombinase (residues 139-1 90) to afford a new 55-residue protein, GGH(Hin 139-1 90). This protein is capable of binding DNA at four 13 base pair sites (termed hixL and secondary). CuDGGH(Hin139-190) in the presence of excess hydrogen peroxide and sodium ascorbate cleaves DNA predominantly at one of the four Hin binding sites. We report here that, in the presence of Ni(OAc), and monoperoxyphthalic acid, the sequence specificity and efficiency of the DNA cleavage by GGH(Hin139-190) are remarkably ...
The iron-binding and oxygen-activating domain of the natural product bleomycin [pyrimidoblamic acid-...
It has long been known that type II topoisomerases require divalent metal ions in order to cleave DN...
Amino terminal peptides of the general form Gly-Gly-His have been used to introduce single sites of ...
We recently designed a sequence-specific DNA-cleaving metalloprotein consisting wholly of naturally ...
A 55-residue protein containing the DNA binding domain of Hin recombinase, residues 139-190, with th...
We report the design and chemical synthesis of a sequence-specific DNA-cleaving protein consisting w...
A unique tripeptide based on the sequence ($\delta$)-Orn-Gly-His was created to circumvent the limit...
DNA cleavage by synthetic tripeptides of the form NH 2-Xaa-Xaa-His-CONH2 was investigated in the pre...
With the goal of developing artificial nucleases for DNA hydrolysis, metal-coordinating peptides hav...
Chapter I: Affinity-Cleaving Studies Examining the Orientation and Specificity of the DNA-Binding Do...
NOTE: Text or symbols not renderable in plain ASCII are indicated by [...]. Abstract is included in ...
Interaction of metal complexes with nucleic acids is currently attracting wide attention due to thei...
The study presented herein utilizes the DNA cleavage activity of Ni(II)•Xaa 1-Xaa2-His metallopeptid...
It has long been known that type II topoisomerases require divalent metal ions in order to cleave DN...
DNA can be oxidatively cleaved by copper complexes of the ATCUN peptide (amino terminal Cu(II)- and ...
The iron-binding and oxygen-activating domain of the natural product bleomycin [pyrimidoblamic acid-...
It has long been known that type II topoisomerases require divalent metal ions in order to cleave DN...
Amino terminal peptides of the general form Gly-Gly-His have been used to introduce single sites of ...
We recently designed a sequence-specific DNA-cleaving metalloprotein consisting wholly of naturally ...
A 55-residue protein containing the DNA binding domain of Hin recombinase, residues 139-190, with th...
We report the design and chemical synthesis of a sequence-specific DNA-cleaving protein consisting w...
A unique tripeptide based on the sequence ($\delta$)-Orn-Gly-His was created to circumvent the limit...
DNA cleavage by synthetic tripeptides of the form NH 2-Xaa-Xaa-His-CONH2 was investigated in the pre...
With the goal of developing artificial nucleases for DNA hydrolysis, metal-coordinating peptides hav...
Chapter I: Affinity-Cleaving Studies Examining the Orientation and Specificity of the DNA-Binding Do...
NOTE: Text or symbols not renderable in plain ASCII are indicated by [...]. Abstract is included in ...
Interaction of metal complexes with nucleic acids is currently attracting wide attention due to thei...
The study presented herein utilizes the DNA cleavage activity of Ni(II)•Xaa 1-Xaa2-His metallopeptid...
It has long been known that type II topoisomerases require divalent metal ions in order to cleave DN...
DNA can be oxidatively cleaved by copper complexes of the ATCUN peptide (amino terminal Cu(II)- and ...
The iron-binding and oxygen-activating domain of the natural product bleomycin [pyrimidoblamic acid-...
It has long been known that type II topoisomerases require divalent metal ions in order to cleave DN...
Amino terminal peptides of the general form Gly-Gly-His have been used to introduce single sites of ...