Effects of curcumin on the acetylation of histone H3, P53 and the proliferation of NB4 cells

目的研究姜黃素對NB4細胞組蛋白H3和非組蛋白P53的乙酰化和細胞增殖的作用,探討姜黃素抗白血病機制。方法應用不同濃度(50,25,12.5,6.25,3.125μmol/L)的姜黃素作用NB4細胞不同時間(0,4,8,12,24h),MTT法測定姜黃素對NB4細胞增殖的影響,Western blot法檢測乙酰化組蛋白H3和乙酰化P53的水平。結果姜黃素以時間和劑量依賴方式抑制NB4細胞增殖,在24h和36h的IC50值分別為40μmol/L和25μmol/L;能明顯上調組蛋白H3的乙酰化水平,促進P53的表達和P53的乙酰化。結論姜黃素具有去乙酰化酶抑制劑作用,能上調組蛋白H3乙酰化水平,促進腫瘤抑制因子P53表達和活化,抑制白血病細胞增殖。OBJECTIVE: To investigate the effects of curcumin on the acetylation of histone H3, P53 and the proliferation of NB4 cells. METHODS: The total protein was extracted from NB4 cells treated without or with different concentrations of curcumin ( 50, 25, 12.5, 6.25 and 3.125 micromol/L) for different time (0, 4, 8, 12, 24 h). Western blot analysis was performed to determine the levels of acetylated histone H3, P53 and acetylated P53. MTT assay was performed to examine the growth inhibition effect of curcumin on NB4 cells. RESULTS: Curcumin could inhibit the proliferation of NB4 cells in a time- and dose- dependent manner, with the IC50 at 24 h and 36 h of 40 micromol/L and 25 micromol/L, respectively. The levels of histone H3 acetylation, P53 expression and P53 acetylation were increased obviously. CONCLUSION: Curcumin functions as a deacetylase inhibitor,which could increase the level of acetylated histone H3, enhance the expression and activity of tumor suppressor P53, and inhibit the proliferation of NB4 cells.link_to_subscribed_fulltex