大鼠類Alu重復序列的克隆及其在染色體DNA突變分析中的應用

A rat Alu like repetitive sequence (RALRS 1) was cloned from rat low Cot DNA library. The cloned RALRS 1 was sequenced and found to be 283 bp in length containing highly repeated AG, AGG, and AGGG sequences. The copy number of RALRS 1sequence was around 150 000～330 000 per rat haploid genome. A synthesized 28 mer oligo nucleotide probe, e.g. (AGGG) 7 was used in the DNA fingerprinting analysis and resulted in a distinct banding pattern. Additionally, the differences of the banding pattern were seen between normal and tumor tissue in rat. This probe should be useful for the gene mutation caused by various factors in rat