High resolution fluorescence studies of metabolic pathways of DNA and oligonucleotide adducts from polycyclic aromatic compounds

The application of the laser-based solid state fluorescence technique fluorescence line-narrowing spectroscopy (FLNS) to the metabolic pathways of DNA and oligonucleotide adducts from benzo(a) pyrene (B(a) P), and 7,12-dimethylbenz(a) anthracene (DMBA) is described. Fluorescence line-narrowed (FLN) spectra of a variety of synthetic model adducts of B(a) P and DMBA expected from one-electron oxidation mechanism are presented. The depurinated adduct in the urine and feces from mouse exposed (in vivo) to B (a) P, depurinated adducts from MC-induced microsome- and horseradish peroxidase-catalazed binding (in vitro) of B (a) P to DNA, and from mouse skin DNA (in vivo) are investigated by the analysis of the FLN spectra obtained from these samples. Similar depurinated DMBA adducts formed with DNA (in vitro) are also identified by FLNS.;In addition, the combination of FLNS at 4.2 K with non-line narrowed (S[subscript]2 ≥ts S[subscript]0 laser excited) fluorescence spectroscopy at 77 K is employed to characterize spatial conformations of adducts formed from (±)-anti-BPDE and single- and double-stranded d(ATATGTATA) or d(CACATGTACAC). Detailed comparisons of the FLN spectra and broad fluorescence spectra obtained under non-line narrowed conditions of the oligonucleotide adducts in solid water and glass host are made and interpreted