Rapid communication: Linkage and physical mapping of the porcine melanocortin-4 receptor (MC4R) gene

Genus and Species. Sus scrofa. Locus. Pig Melanocortin-4 Receptor (MC4R) gene. Source and Description of Primers. Primers were designed from well-conserved sequence regions between the human and rat MC4R genes (Genbank accession no. S77415 and U67863, respectively). The primers were used to amplify approximately 760 bp of the porcine MC4R gene from genomic DNA. The sequence of the PCR product showed 92.2 and 97.6% identities at the nucleotide and amino acid levels, respectively, to the corresponding human sequence. The porcine MC4R sequence has been submitted to GenBank (accession no. AF087937). Using this sequence, pig-specific primers were designed