Anti-cancer effects of Palladium complexes on gastric cancer cell line (AGS)

Introduction: Chemotherapy drugs are used to treat of various types of neoplasms. In doing so, such drugs leave many side effects. Furthermore, in recent years, several attempts have been made to develop drugs based on platinum or palladium which have the low toxicity and is more sensitive to the drug-resistant diseases. Objective: To evaluate the effects of newly synthetized palladium complexes as anticancer drugs on gastric cancer cell line (AGS). Materials & Methods: In this study, gastric cancer cell line (AGS) was purchased from Pasteur Institute, Iran and cultivated in RPMI 1640 and then, the cytotoxic effects of various concentrations of newly synthesized complexes were evaluated by clonogenic assay and acridine orange/ ethedium bromide staining. Results: The results showed that the new complexes have different effects in concentration-dependent manner so that complexe1 ≥ 0.1 µg /ml, complexe2 ≥ 0.05 µg /ml and complexe3 ≥ 1 µg /ml lead to cell death by apoptosis and necrosis. Comparison of the number of colonies formed after treatment with concentrations of palladium complexes showed significant differences, compared with control (P