Application of the Direct Colony TLC for Identification of Phytopathogenic Bacteria (II) Chromatographic Profile of Erwinia and Pseudomonas spp.

A rapid identification of phytopathogenic bacteria was available at species level by the direct colony thin-layer chromatography. Distinct difference was observed in chromatographic profile between Emoirza’a chrysanthemi and E. carotovora subsp. carotovora. In the case of Erwinia carotovora subsp. carotovora, a characteristic spot at Rf 0.57 appeared concomitantly with a common spot at 0.62. The chromatograms of Pseudomonas spp. were divided into three major types. The chromatograms of P. gladioli pv. gladioli, P. glumae, P. plantarii, P. caryophylli and P. cepacia resembled each other. This chromatographic profile was clearly different from those of other pseudomonads and designated as a Cepacia-type. In the case of P. solanacearum, a characteristic spot appeared at Rf 0.54 and this profile was named as a Solanacearum-type. The profile of remaining pseudomonads was simple and similar each other, and was designated as a Syringae-type. A practical usefulness of this easy method was certified also in this experiment