Deoxyribonuclease and protease in virus-diseased silkworms

Variations of deoxyribonuclease and protease activities were investigated at varying intervals after infecting virus. It was demonstrated that the activation of protease is relatively small within 2 or 3 days after infection and becomes much higher in 4 or 5 days. Deoxyribonuclease, however, is activated only in the latter half of virosis development. The action of these enzymes i n worms was further measured a few hours after treating with inducers and virus. It was established that the activity of protease as well as deoxyribonuclease is augmented by the injection of hydroxylamine, hydrogen peroxide, potassium nitrite, acetoxime, sodium carbonate and polyhedra.多角体バイラス蛋白の合成は蚕の幼虫体内に於ける核酸の仲介によつて進行するものと想定せられるが,前報記載の如くリボヌクレアーゼの作用力はバイラスの成長に伴い比較的低度の賦活を示したに過ぎなかつた.しかし蚕の組織に於ては多角体結晶が多くの場合細胞核内に形成されることから,バイラス病の発生は先ず染色体乃至病毒粒子の外側をなす蛋白質が活性化された蛋白酵素によつて水解され,次に内部のデオキシリボ核酸がヌクレアーゼにより或程度分解せられた後に増殖することによつて開始されるものと推測せられる.従つて本報では蚕体内のデオキシリボヌクレアーゼに対し病毒の接種或は誘発試薬の処理が及ぼす影響をプロテアーゼの作用と併行して観察してみた