Molecular Analysis of ITS Region and Antibacterial Activities of Stereum hirsutum

Stereum hirsutum is one of the multicolored medicinal mushrooms belongs to Stereaceae, Basidiomycota. Identification of this mushroom is often confused with Trametes versicolor, the so called “true” Turkey tail. Because of that we studied ITS (internal transcribe spacer) region and PCR analysis to confirm the species. According to the taxonomic report of BLAST, obtained result of sequence having 558 bp length and 179.45 kDa weight indicates that the studied fungus was S. hirsutum which similar to NCBI database (maximum and total score 1048, query coverage 100% and maximum identity 99% compare to consensus reference strain dd08031, No. gi|226346602| FJ810148.1). BLAST also computes a pairwise alignment between the query and database sequences searched. In neighbor joining tree, sequence of studied sample was placed with consensus other similar sequences of database mentioning the taxonomic name and sequence title that also indicate the studied sample was supposed to be S. hirsutum species. The findings of optical density referred that the filtrates of S. hirsutum was found to inhibit both Gram positive and Gram negative bacteria. Bacillus subtilis and Pseudomonas aeruginosa are highly inhibited by the culture filtrates. The lowest inhibition was found for Klebsiella pneumoniae but after 24 hours of incubation inhibition rate was accelerated. The results obtained from the paper disc method, all of 5 bacteria were less or more inhibited by the 3 types of culture filtrates. Liquid culture and water extracts were demonstrated the lowest and highest inhibition zones of studied bacteria, respectively. In liquid culture, the highest and lowest inhibitions were found to be seen for Staphylococcus aureus and Escherichia coli, respectively. In case of water and ethanol extracts, P. aeruginosa, S. aureus and B. subtilis were highly inhibited, respectively as well as E. coli and K. pneumoniae were less inhibited in all extracts