Analysis of second harmonic generation polarization profiles: an attempt to devise a complete three-dimensional model

We report the first attempt to build a three dimensional model of the polarization modulated second harmonic generation and emission dynamics from collagen fibrils under realistic conditions. Our analytical model is constructed by integration of previous knowledge on the stimulation of a second harmonic polarization in a non-centrosymmetric cylindrical target, and on the propagation of a resultant second harmonic disturbance. The application of our paradigm to actual biological targets allows one to retrieve their spatial orientation, mutual organization and inner configuration, which holds great potential to develop biological investigations and theragnostic applications.