PRIL_A, PRIL_B and PRIL_C: Pigeonpea Recombinant Inbred Line Mapping Populations Segregating for Resistance to Fusarium Wilt and Sterility Mosaic Disease

Pigeonpea [Cajanus cajan (L) Millsp] bi-parental populations segregating for various traits of interest are being developed. The three most advanced populations, named PRIL_A, PRIL_B and PRIL_C (Pigeonpea Recombinant Inbred Line, population A, B and C) have reached F6 generation. PRIL_A: derived from the cross ICPB 2049 x ICPL 99050 segregates for fusarium wilt (FW), 329 lines. PRIL_B: derived from the cross ICPL 20096 x ICPL 332 segregates for FW and sterility mosaic disease (SMD), 342 lines. PRIL_C: derived from the cross ICPL 20097 × ICP 8863 segregates for SMD, 366 lines. Marker genotyping of the parental lines, however, showed low level of genetic variation. After screening over 4,616 (3,000 simple sequence repeats (SSRs) and 1,616 single nucleotide polymorphism (SNPs)) markers on parental genotypes of each mapping population, a total of 159 (104 SSRs and 55 SNPs), 80 (52 SSRs and 28 SNPs) and 157 (143 SSRs and 14 SNPs) markers were found polymorphic for ICPB 2049 vs ICPL 99050, ICPL 20096 vs ICPL 332 and ICPL 20097 vs ICP 8863, respectively. The polymorphic markers will be used for constructing genetic linkage maps. The populations will be screened for FW and SMD in 2012-13, while marker-trait association analysis will also be conducted to understand the genetic basis of resistance to these diseases. Further selection from the above indicated mapping populations during 2011-12, on an effort initiated in 2010, resulted in 28 lines with high yield (up to 2.4 t/ha) and disease resistanc