Identification of the Male-Produced Aggregation Pheromone of the Four-Spotted Coconut Weevil, Diocalandra frumenti

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/acs.jafc6b04829[EN] The four-spotted coconut weevil, Diocalandra frumenti Fabricius (Coleoptera: Dryophthoridae), is a small weevil found attacking economically important palm species, such as coconut, date,, oil, and Canary palms. Given the scarcity of detection and management tools for this pest, the availability of a pheromone to be included in trapping protocols would be a crucial advantage. Previous laboratory experiments showed evidence for aggregation behavior; thus, our main goal was to identify the aggregation pheromone in this species. The volatile profile of D. frumenti individuals was studied by aeration and collection of effluvia in Porapak-Q and also by solid phase microextraction (SPME) techniques. Moreover, solvent extraction of previously frozen crushed individuals was also performed. All resulting extracts and SPME fibers were analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The comparison of male and female samples provided the candidate compound, 5ethyl-2,4-dimethy1-6,8-dioxabicyclo [3.2.1]octane (multistriatin), whose biological activity was evaluated in olfactometer and field assays.This work received funding from Direccion General de Agricultura del Gobierno de Canarias (Spain).Vacas González, S.; Navarro Fuertes, I.; Seris, E.; Ramos, C.; Hernández Suárez, E.; Navarro-Llopis, V.; Primo Millo, J. (2017). Identification of the Male-Produced Aggregation Pheromone of the Four-Spotted Coconut Weevil, Diocalandra frumenti. Journal of Agricultural and Food Chemistry. 65(2):270-275. doi:10.1021/acs.jafc6b04829S27027565