First Proposed Panels on Acute Leukemia for Four-Color Immunophenotyping by Flow Cytometry from the Brazilian Group of Flow Cytometry-GBCFLUX

Multiparameter flow cytometry is a highly sensitive, fast, and specific diagnostic technology with a wide range of applicability in hematology. Although well-established eight-color immunophenotyping panels are already available, most Brazilian clinical laboratories are equipped with four-color flow cytometer facilities. Based on this fact, the Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria de Fluxo, GBCFLUX) for standardization of clinical flow cytometry has proposed an antibody panel designed to allow precise diagnosis and characterization of acute leukemia (AL) within resource-restricted areas. Morphological analysis of bone marrow smears, together with the screening panel, is mandatory for the primary identification of AL. the disease-oriented panels proposed here are divided into three levels of recommendations (mandatory, recommendable, and optional) in order to provide an accurate final diagnosis, as well as allow some degree of flexibility based on available local resources and patient-specific needs. the proposed panels will be subsequently validated in an interlaboratory study to evaluate its effectiveness on the diagnosis and classification of AL. (Assoc editor comm. 2). (c) 2015 International Clinical Cytometry SocietyHosp Amaral Carvalho, Lab Citometria Fluxo Hemonucleo Reg Jau, São Paulo, BrazilFleury Grp, Div Hematol, São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP EPM, Div Hematol & Blood Transfus Med, Escola Paulista Med, São Paulo, BrazilBrazilian Natl Canc Inst INCa, Canc Res Ctr, Rio de Janeiro, BrazilUniv Fed Rio Grande do Norte, BR-59072970 Natal, RN, BrazilUniv Estadual Campinas, São Paulo, BrazilUniv Fed Rio de Janeiro, Pediat Inst IPPMG, Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Rio de Janeiro, BrazilLab Diagnost Amer, Rio de Janeiro, BrazilUniv Fed Santa Catarina, BR-88040900 Florianopolis, SC, BrazilHosp Israelita Albert Einstein, São Paulo, BrazilCtr Hematol, São Paulo, BrazilLab Diagnost Amer, São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP EPM, Div Hematol & Blood Transfus Med, Escola Paulista Med, São Paulo, BrazilWeb of Scienc