Coacervate whey protein improves inflammatory milieu in mice fed with high-fat diet

Background: Functional foods with bioactive properties may help in treat obesity, as they can lead to a decreased risks of inflammatory diseases. the aim of this study was to investigate the effects of chitosan coacervate whey protein on the proinflammatory processes in mice fed with high-fat diet.Methods: Mice were divided into two groups receiving either a normolipidic or high-fat diet; the animals in each of the two diet groups were given a diet supplement of either coacervate (gavage, 36 mg protein/kg of body weight) or tap water for four weeks [groups: normolipidic diet plus water (C); normolipidic diet and coacervate (CC); high-fat diet and water (H); and high-fat diet and coacervate (HC)].Results: the high-fat diet promoted inflammation, possibly by decreased adiponectin/sum of adipose tissues ratio and increased phosphorylation of NF-kappa B p50. in HC we observed a positive correlation between IL-10 and TNF-alpha in mesenteric adipose tissue, retroperitoneal adipose tissue and liver tissue. We also observed a positive correlation between lipopolisaccharide with IL-10 in the liver tissue.Conclusions: High-fat diet treatment promoted metabolic alterations and inflammation, and chitosan coacervate whey protein modulated inflammatory milieu.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Fisiol, Disciplina Fisiol Nutr, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Ciencias & Tecnol, Sao Jose Dos Campos, SP, BrazilUniversidade Federal de São Paulo, Dept Fisiol, Disciplina Fisiol Nutr, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Ciencias & Tecnol, Sao Jose Dos Campos, SP, BrazilFAPESP: 2011/04310-9Web of Scienc