AGH is a new hemoglobin alpha-chain fragment with antinociceptive activity
- Ribeiro, Natalia M.
- Toniolo, Elaine F.
- Castro, Leandro M.
- Russo, Lilian C.
- Rioli, Vanessa
- Ferro, Emer S.
- Dale, Camila S.
DOIAbstract
Limited proteolysis of certain proteins leads to the release of endogenous bioactive peptides. Hemoglobin-derived peptides such as hemorphins and hemopressins are examples of intracellular protein-derived peptides that have antinociceptive effects by modulating G-protein coupled receptors activities. in the present study, a previously characterized substrate capture assay that uses a catalytically inactive form of the thimet oligopeptidase was combined with isotopic labeling and mass spectrometry in order to identify new bioactive peptides. Indeed, we have identified the peptide AGHLDDLPGALSAL (AGH), a fragment of the hemoglobin alpha-chain, which specifically bind to the inactive thimet oligopeptidase in the substrate capture assay. Previo...
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