Gradual strenuous running regimen predisposes to osteoarthritis due to cartilage cell death and altered levels of glycosaminoglycans

Objective: To investigate the hypothesis that strenuous running is a predisposing factor for osteoarthritis.Design: Wistar rats were divided into two groups: a control group (CG) and a trained group (TG). the TG underwent a strenuous treadmill running training regimen of controlled intensity, exhibiting progressively improvement of fitness over 12 weeks, running at least 55 km during this period and finally performing an ultra-endurance running exercise to exhaustion. After this period, rats from both groups were euthanized and their knees removed. the articular cartilage was dissected and submitted to histomorphometrical, histomorphological, and immunohistochemical analyses evaluating cell death pathway (caspase-3 and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick 'end labeling (TUNEL)) and inflammatory cytokines [interleukin-1 alpha (IL-1 alpha) and tumor necrosis factor-alpha (TNF-alpha)]. in addition, the tissues were analyzed regarding the types and the content of glycosaminoglycans.Results: the TG knee joints exhibited increase in the number of chondrocytes and chondrocyte clusters, as well as significantly increased levels of caspase-3, a protein involved in apoptosis, and of inflammatory cytokines IL-1 alpha and TNF-alpha. in addition, histologically higher grades of osteoarthritis (Osteoarthritis Research Society International - OARSI grading), and significantly decreased levels of chondroitin sulfate and hyaluronic acid. Knee cartilage thickness and TUNEL did not significantly differ between the two groups.Conclusions: the articular cartilage of rats subjected to a strenuous running regimen of controlled intensity exhibited molecular and histological characteristics that are present in osteoarthritis. (C) 2013 Osteoarthritis Research Society International. Published by Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Escola Paulista Med, Dept Orthopaed & Traumatol, BR-04038032 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Morphol & Genet, BR-04038032 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Program Mol Biol, BR-04038032 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Orthopaed & Traumatol, BR-04038032 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Morphol & Genet, BR-04038032 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Program Mol Biol, BR-04038032 São Paulo, BrazilWeb of Scienc