Optimizing dentin bond durability: Control of collagen degradation by matrix metalloproteinases and cysteine cathepsins

Objectives. Contemporary adhesives lose their bond strength to dentin regardless of the bonding system used. This loss relates to the hydrolysis of collagen matrix of the hybrid layers. the preservation of the collagen matrix integrity is a key issue in the attempts to improve the dentin bonding durability.Methods. Dentin contains collagenolytic enzymes, matrix metalloproteinases (MMPs) and cysteine cathepsins, which are responsible for the hydrolytic degradation of collagen matrix in the bonded interface.Results. the identities, roles and function of collagenolytic enzymes in mineralized dentin has been gathered only within last 15 years, but they have already been demonstrated to have an important role in dental hard tissue pathologies, including the degradation of the hybrid layer. Identifying responsible enzymes facilitates the development of new, more efficient methods to improve the stability of dentin-adhesive bond and durability of bond strength.Significance. Understanding the nature and role of proteolytic degradation of dentin-adhesive interfaces has improved immensely and has practically grown to a scientific field of its own within only 10 years, holding excellent promise that stable resin-dentin bonds will be routinely available in a daily clinical setting already in a near future. (c) 2012 Academy of Dental Materials. Published by Elsevier B.V. All rights reserved.NIDCRAcademy of FinlandUniv Oulu, Inst Dent, Oulu 90014, FinlandOulu Univ Hosp, Oulu, FinlandNord Inst Dent Mat NIOM, Oslo, NorwayUniv Bandeirante São Paulo, Biomat Res Grp, São Paulo, BrazilUniv Trieste, Unit Dent Sci & Biomat, Dept Med Sci, I-34127 Trieste, ItalyIGM CNR, Unit Bologna, IOR, Bologna, ItalyRizzoli Orthoped Inst, Ramses Lab, Lab Immunorheumatol & Tissue Regenerat, Bologna, ItalyRizzoli Orthoped Inst, Ramses Lab, Cell Biol Lab, Bologna, ItalyUniv Mogi das Cruzes, Ctr Interdisciplinar Invest Bioquim, São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Disciplina Biol Mol, São Paulo, BrazilUniv Florida, Coll Dent, Restorat Dent Sci Operat Div, Gainesville, FL USAUniv Turku, Inst Dent, Dept Prosthet Dent, Turku, FinlandTurku Univ Hosp, FIN-20520 Turku, FinlandUniv Western Ontario, London, ON, CanadaUniv British Columbia, Div Biomat, Dept Oral Biol & Med Sci, Vancouver, BC V5Z 1M9, CanadaMed Coll Georgia, Sch Dent, Dept Endodont, Augusta, GA 30912 USAMed Coll Georgia, Sch Dent, Dept Oral Biol, Augusta, GA 30912 USAUniversidade Federal de São Paulo UNIFESP, Disciplina Biol Mol, São Paulo, BrazilNIDCR: de 015306Web of Scienc